Simple. Powerful. Fast.
Immune System Monitoring with CyTOF
You need answers from every cell in each precious patient sample, and you need them quickly, reliably.
Mass cytometry, powered by CyTOF® technology, can expedite COVID-19 therapeutic and vaccine research studies with best-in-class, highly multiplexed, longitudinal immune monitoring capability.
Faster and more cost-efficient than single-cell RNA-seq, CITE-seq or AbSeq, mass cytometry also provides deeper resolution of immune cell phenotype and function than any flow cytometry or spectral cytometry system.
And CyTOF does it all in a single tube.Get the flyer
In this presentation, Marcelo B. Sztein, MD, shows examples of his group’s use of mass cytometry to help advance the fields of vaccine development and pathogen-host interactions. He provides an in-depth look at his studies of Salmonella typhi (S. typhi), the causative agent of typhoid fever, an infectious disease of great public health importance.
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Jump-start your SARS-CoV-2 vaccine and COVID-19 therapeutic research with the Maxpar Direct Immune Profiling System.
- Systems-level panel reduces bias and helps you find the unknown.
- Contextual relationships between immune cell subsets maintained.
- Proven run-to-run reproducibility
Solves multi-site study challenges
- Simply freeze, store and ship samples.
- Easy-to-implement protocols and rapid, bias-free data analysis
- Demonstrated inter-site reproducibility
Panel flexibility and expansion
- 14-plus open channels for custom marker addition
- Maxpar Direct Expansion Panels provide flexible and focused solutions for further customization.
“We chose the Maxpar Direct Immune Profiling Assay for this study* because it provides a robust and standardized solution for comprehensive immune monitoring that is technically easy to execute and harmonize across multiple study sites.”—Adeeb Rahman, PhD
Icahn School of Medicine at Mount Sinai
University of Leuven, Belgium
Professor of Internal Medicine
Director, Yale CyTOF Facility
Associate Dean for Scientific Affairs
Published research and trials using the Maxpar Direct Immune Profiling Assay
National Clinical Trials
- Immunophenotyping Assessment in a COVID-19 Cohort (IMPACC) (NCT04378777)
Sponsor: National Institute of Allergy and Infectious Diseases (NIAID); 10 participating institutions in North America
- In-Depth Immunological Investigation of COVID-19. (COntAGIouS) (NCT04327570)
Sponsor: Universitaire Ziekenhuizen Leuven
- Antidepressant Trial with P2X7 Antagonist JNJ-54175446 (ATP) (NCT04116606)
Clinical trials using mass cytometry in vaccine research as of September 30, 2020.Source:clinicaltrials.gov
|Clinical Trial Name||Sponsor||Start Date||Phase||NCT Number|
|FluPRINT Study: Characterisation of the Immune and Transcriptional Response to LAIV||University of Oxford||October 2019||NA*||04222595|
|CVD 38000: Study of Responses to Vaccination with Typhoid and/or Cholera||University of Maryland||November 2018||4||03705585|
|High Dose Flu Vaccine in Treating Children Who Have Undergone Donor Stem Cell Transplant||Vanderbilt-Ingram Cancer Center/ National Cancer Institute||September 2016||2||02860039|
|Investigating Enteric Fever—Salmonella Typhi and Paratyphi Challenge Study||University of Oxford, Oxford Vaccine Group||December 2014||NA||02192008|
|Genetic and Environmental Factors in the Response to Influenza Vaccination (SLVP028)||Stanford University||October 2014||1||03088904|
|The Role of CD4+ Memory Phenotype, Memory, and Effector T Cells in Vaccination and Infection (SLVP030)||Stanford University||September 2014||1||03453801|
|CVD 37000: Immunity and Microbiome Studies at Intestinal and Systemic Sites in Ty21a Vaccinated Adults||University of Maryland||October 2013||4||03970304|
|Study of a New MVA Vaccine for Hepatitis C Virus||ReiThera Srl||December 2010||1||01296451|
|Blood Donor CVD 5000||University of Maryland||January 2004||4||03971669|
Key Publications Applying Mass Cytometry for Vaccine Research
- Brodin, P. “Technologies for assessing vaccine responses in the very young.” Current Opinion in Immunology 65 (2020):28–31.
- Chng, M.H.Y. et al. “Large-scale HLA tetramer tracking of T cells during dengue infection reveals broad acute activation and differentiation into two memory cell fates.” Immunity 51 (2019): 1,119–1,135.
- Lingblom, C.M.D. et al. “Baseline immune profile by CyTOF® can predict response to an investigational adjuvanted vaccine in elderly adults.” Journal of Translational Medicine 16 (2018): 153.
- Lucchesi, S. et al. “From bivariate to multivariate analysis of cytometric data: overview of computational methods and their application in vaccination studies.” Vaccines 8 (2020): 138.
- Newell E.W. et al. “Combinatorial tetramer staining and mass cytometry analysis facilitate T-cell epitope mapping and characterization.” Nature Biotechnology 31 (2013):623–629.
- Palgen, J-L. et al. “Innate and secondary humoral responses are improved by increasing the time between MVA vaccine immunizations.” npj Vaccines (2020): 24.
- Palgen, J-L. et al. “NK cell immune responses differ after prime and boost vaccination.” Journal of Leukocyte Biology (2019): 1,055–1,073.
- Palgen, J-L. et al. “Prime and boost vaccination elicit a distinct innate myeloid cell immune response.” Scientific Reports 8 (2018): 3,087.
- Reeves, P.M. et al. “Application and utility of mass cytometry in vaccine development.” FASEB Journal 32: (2018): 5–15.
- Rudolph, M.E. et al. “Diversity of Salmonella Typhi-responsive CD4 and CD8 T cells before and after Ty21a typhoid vaccination in children and adults.” International Immunology (2019): 315–333.
- Tomic, A. et al. “SIMON, an automated machine learning system, reveals immune signatures of influenza vaccine responses.” The Journal of Immunology 1203 (2019): 749–759.
- Tomic, A. et al. “The FluPRINT dataset, a multidimensional analysis of the influenza vaccine imprint on the immune system.” Scientific Data 6 (2019): 214.